Decellularization of lung tissue and analysis of its differentiative potential on bone marrow mesenchymal stem cells of rat
DOI:
https://doi.org/10.22034/JATE.2015.12Keywords:
Decellularization, SDS, Differentiation, CC10, SPDAbstract
Background: Pulmonary diseases are one of the most common causes of mortality worldwide. Lung transplantation is the only curative treatment in some cases. Very few lungs are available for transplantation and the 5-year survival after lung transplantation is 50%. Transplant recipients require immunosuppressant therapy in that’s time. Production of engineered lung tissue using stem cells is a promising approach. Extraction of natural ECM or decellularization of lung and application of it in tissue engineering is one of the most important strategies in this regard. It can preserve the natural characteristics of the ECM and would leads to the removal of the immunogen agents (MHC I, II) and allows reconstruction of graft.
Objective: we have decellularized rat lung and cultured bone marrow stem cells on it to evaluate its differentiative potential.
Methods: In this study along with extraction of rat lung, its femur and tibia bones were isolated for extraction of mesenchymal stem cells. Lung tissue was decellularized using SDS detergent. SEM and H&E staining used to assay decellularization. Mesenchymal stem cells were seed on the decellularized tissue sections and immunocytochemistry for CC10 and SPD performed on these cells after 21 days.
Results: H&E slides of decellularized tissue did not show any cells. In electron microscope images of decellularized tissue alveolar structure was well maintained. Immunocytochemistry assay showed differentiation of seeded mesenchymal cells toward lung tissue cells.
Conclusion: Decellularization take placed without any significant change in tissue structure. Decellularized tissue induced differentiation of bone marrow stem cells toward lung epithelial cells so natural ECM saved relatively.
References
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3. Badylak SF, Weiss DJ, Caplan A, Macchiarini P. Engineered whole organs and complex tissues. The Lancet, 2012. 379(9819): p. 943-952.
4. Jungebluth P, Moll G, Baiguera S, Macchiarini P. Tissue-engineered airway: a regenerative solution. Clinical Pharmacology & Therapeutics, 2011. 91(1): p. 81-93.
5. Jungebluth P, Bader A, Baiguera S, Moller S, Jaus M, Lim ML, Fried K, Kjartansdóttir KR, Go T, Nave H, Harringer W, Lundin V, Teixeira AI, Macchiarini P. The concept of in vivo airway tissue engineering. Biomaterials, 2011. 33(17): p. 4319-4326
6. Haykal S, Soleas JP, Salna M, Hofer SO, Waddell TK. Evaluation of the structural integrity and extracellular matrix components of tracheal allografts following cyclical decellularization techniques: comparison of three protocols. Tissue Engineering Part C: Methods, 2012. 18(8): p. 614-623.
7. Haag J, Baiguera S, Jungebluth P, Barale D, Del Gaudio C, Castiglione F, Bianco A, Comin CE, Ribatti D, Macchiarini P. Biomechanical and angiogenic properties of tissue-engineered rat trachea using genipin cross-linked decellularized tissue. Biomaterials, 2012. 33(3): p. 780-789.
8. Baiguera S, Del Gaudio C, Jaus MO, Polizzi L, Gonfiotti A, Comin CE, Bianco A, Ribatti D, Taylor DA, Macchiarini P. Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues. Biomaterials, 2012. 33(14): p. 3662-3672
9. Fishman JM, De Coppi P, Elliott MJ, Atala A, Birchall MA, Macchiarini P. Airway tissue engineering. Expert opinion on biological therapy, 2011. 11(12): p. 1623-1635.
10. Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, Taylor DA. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nature medicine, 2008. 14(2): p. 213-221.
11. Badylak SF. The extracellular matrix as a biologic scaffold material. Biomaterials, 2007. 28(25): p. 3587-3593.
12. Hoshiba T, Lu H, Kawazoe N, Chen G. Decellularized matrices for tissue engineering. Expert opinion on biological therapy, 2010. 10(12): p. 1717-1728.
13. Tavassoli A, Shahabipour F, Mahdavi-shahri N, Moghadam-matin M, Fereidoni M. In vitro experimental study of interactions between blastema tissue and three-dimensional matrix derived from bovine cancellous bone and articular cartilage. JOURNAL OF CELL, 2010.
14. Mahdavi-Shahri N, Akbarzadeh-nika M, Moghadam-matin M, Fereidoni M, Lari R. The Histological Study of the Interactions between Rabbit Decellularized Esophagus Scaffold and the Blastema Tissue Obtained from the Pinna of New Zealand White Rabbit. Journal of Isfahan Medical School, 2014. 31(261).
15. Ott HC, Clippinger B, Conrad C, Schuetz C, Pomerantseva I, Ikonomou L, Kotton D, Vacanti JP .Regeneration and orthotopic transplantation of a bioartificial lung. Nature medicine, 2010. 16(8): p. 927-933.
16. Mendez JJ, Ghaedi M, Steinbacher D, Niklason LE. Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds. Tissue Engineering Part A, 2014. 20(11-12): p. 1735-1746.
17. Alizadeh A, Dashtnavard H, Tiraihi MT. The influence of lithium chloride on induction of bone marrow stromal cells into neuronal phenotype.Medical daneshvar 2009;16(79):51-56.
18. Pedram MS, Soleimani M, Shafii D, Marjanmehr S, Nasiri Z. Harvesting of bone marrow mesenchymal stem cells from live rats and the in vitro differentiation of bone marrow mesenchymal stem cells into neuron-like cells. International Journal of Veterinary Research, 2010. 4(1).
19. Gong X, Sun Z, Cui D, Xu X, Zhu H, Wang L, Qian W , Han X . Isolation and characterization of lung resident mesenchymal stem cells capable of differentiating into alveolar epithelial type II cells. Cell biology international, 2014. 38(4): p. 405-411.
20. Gilbert TW, Sellaro TL, Badylak SF. Decellularization of tissues and organs. Biomaterials, 2006. 27(19): p. 3675-3683.
21. Shannon JM, Gebb SA, Nielsen LD. Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture. Development, 1999. 126(8): p. 1675-1688.
22. Kuttan R, Spall RD, Duhame RC, Sipes IG, Meezan E, Brendel K. Preparation and composition of alveolar extracellular matrix and incorporated basement membrane. Lung, 1981. 159(1): p. 333-345.
23. Tzouvelekis A, Ntolios P, Bouros D. Stem cell treatment for chronic lung diseases. Respiration, 2013. 85(3): p. 179-192.
24. Hoshiba T, Lu H, Kawazoe N, Chen G. Decellularized matrices for tissue engineering. Expert opinion on biological therapy, 2010. 10(12): p. 1717-1728.
25. Mahdavi-Shahri N, Akbarzadeh-nika M, Moghadam-matin M , Fereidoni M, Lari R. The Histological Study of the Interactions between Rabbit Decellularized Esophagus Scaffold and the Blastema Tissue Obtained from the Pinna of New Zealand White Rabbit. Journal of Isfahan Medical School, 2014. 31(261).
26. Ott HC, Clippinger B, Conrad C, Schuetz C, Pomerantseva I, Ikonomou L, Kotton D, Vacanti JP. Regeneration and orthotopic transplantation of a bioartificial lung. Nature medicine, 2010. 16(8): p. 927-933
27. Mendez JJ, Ghaedi M, Steinbacher D, Niklason LE. Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds. Tissue Engineering Part A, 2014. 20(11-12): p. 1735-1746.
28. Maghsoudlou P, Totonelli G, Loukogeorgakis SP, Eaton S, De Coppi P. A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix. Journal of visualized experiments, 2013(80).
29. Jensen T, Roszell B, Zang F, Girard E, Matson A, Thrall R, Jaworski DM, Hatton C, Weiss DJ, Finck C. A rapid lung de-cellularization protocol supports embryonic stem cell differentiation in vitro and following implantation. Tissue Engineering Part C: Methods, 2012. 18(8): 632-646.
30. Bonvillain RW, Scarritt ME, Pashos NC, Mayeux JP, Meshberger CL, Betancourt AM, Sullivan DE, Bunnell BA. Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor. Journal of Visualized Experiments, 2013(82): p. 50825-50825
31. Tsunooka N, Hirayama S, Medin JA, Liles WC, Keshavjee S, Waddell TK .A novel tissue-engineered approach to problems of the postpneumonectomy space. The Annals of thoracic surgery, 2011. 91(3): p. 880-886.
32. Hoffman AM, Paxson JA, Mazan MR, Davis AM, Tyagi S, Murthy S, Ingenito EP. Lung-derived mesenchymal stromal cell post-transplantation survival, persistence, paracrine expression, and repair of elastase-injured lung. Stem cells and development, 2011. 20(10): p. 1779-1792.
33. Song JJ, Kim SS, Liu Z, Madsen JC, Mathisen DJ, Vacanti JP, Ott HC. Enhanced in vivo function of bioartificial lungs in rats. The Annals of thoracic surgery, 2011. 92(3): p. 998-1006.
34. Ma N, Gai H, Mei J, Ding FB, Bao CR, Nguyen DM, Zhong H. Bone marrow mesenchymal stem cells can differentiate into type II alveolar epithelial cells in vitro. Cell biology international, 2011. 35(12): p. 1261-1266.
35. Jaberansari Z. Decellularized lung matrix as a scaffold for mouse lung stem cells. 2012, University of Minnesota.
36. Nichols JE, Cortiella J. Engineering of a complex organ: progress toward development of a tissue-engineered lung. Proceedings of the American Thoracic Society, 2008. 5(6): p. 723-730.
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Published
2019-12-07
How to Cite
Tarahani Nia, M. ., Alizadeh, A. ., Takhshid, M. A. ., & Sadroddiny, E. . (2019). Decellularization of lung tissue and analysis of its differentiative potential on bone marrow mesenchymal stem cells of rat. The Journal of Applied Tissue Engineering, 2(1), 35–45. https://doi.org/10.22034/JATE.2015.12
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