Metallothionein molecules in sciatic nerve injury and repair

Authors

  • Fatemeh Aghanassir
  • Hassan Aghaei
  • Abbas Ali Imani Fouladi
  • Mohammad Reza Nourani4

DOI:

https://doi.org/10.22034/JATE.2016.15

Keywords:

Metallothionein 1 & 2, oxidative stress, Crush injury, peripheral nerve

Abstract

 

 

Abstract:

Background and purpose: Peripheral nerve injury induces oxidative stress in lesioned neurons. Antioxidants remove excess free radicals and participate in nerve repair. In this study, we assessed the antioxidative role of the metallothionein 1&2 molecules in sciatic nerve repair in rats.

 Materials and methods: The sciatic nerves of 40 rats were crushed and the total mRNA of samples from day 1 and 3 and week 1, 3, 5 post injury was extracted. The expression of the MT-1&2 genes was confirmed by RT-PCR. For immunohistochemistry analysis, the samples were fixed in paraformaldehyde and cut in 20 micrometer slices by cryostat.

Results: The expression of MT-1&2 significantly up-regulated in day 1 and 3 following the crushing of sciatic nerves in comparison with the intact nerves. Immunohistochemistry results also confirmed the protein expression of MT-1.

Conclusion: The metallothionein molecules showed up-regulation in the degeneration process after nerve injury, so it may play an important role in nerve repair.  

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Published

2019-12-08

How to Cite

Aghanassir, F. ., Aghaei, H. ., Imani Fouladi, A. A. ., & Nourani4, M. R. (2019). Metallothionein molecules in sciatic nerve injury and repair. The Journal of Applied Tissue Engineering, 3(1), 25–33. https://doi.org/10.22034/JATE.2016.15

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