Metallothionein molecules in sciatic nerve injury and repair
Background and purpose: Peripheral nerve injury induces oxidative stress in lesioned neurons. Antioxidants remove excess free radicals and participate in nerve repair. In this study, we assessed the antioxidative role of the metallothionein 1&2 molecules in sciatic nerve repair in rats.
Materials and methods: The sciatic nerves of 40 rats were crushed and the total mRNA of samples from day 1 and 3 and week 1, 3, 5 post injury was extracted. The expression of the MT-1&2 genes was conﬁrmed by RT-PCR. For immunohistochemistry analysis, the samples were ﬁxed in paraformaldehyde and cut in 20 micrometer slices by cryostat.
Results: The expression of MT-1&2 signiﬁcantly upregulated in day 1 and 3 following the crushing of sciatic nerves in comparison with the intact nerves. Immunohistochemistry results also conﬁrmed the protein expression of MT-1.
Conclusion: The metallothionein molecules showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair.
Keywords: metallothionein 1 & 2, oxidative stress, Crush injury, peripheral nerve
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