Comparison the differentiate ability of wisdom tooth follicle stem cells into nerve and bone tissue
Background: The ultimate goal of craniomaxillofacial treatment is to achieve bone, nerve, cartilage regeneration with the reconstruction of all the components. The goal of this study was to assess Comparison the differentiate ability of wisdom tooth follicle stem cells into nerve and bone tissue
Methods and Materials: in this experimental study, 3 samples of impacted 3rd molar follicle were used to isolate stem cells. After approved the mesenchymal stem cells, neural differentiation and bone differentiation of cells cultured in standard medium culture for neural differentiation and bone differentiation. Twenty-one days later the differentiation of the stem cells were examined and compared.
Results: Upon examining the gene expression of osteoblasts, osteocalcin, beta Tubulin genes and MAP2 the cultured specimens had statistically desirable osteoblastic and neuroblastic induction.
Conclusion: Results showed that neurogenic and osteogenic differentiating potential was detected in derived stem cell from tooth follicle cells. This study demonstrated that dental follicle can be used in the cellular treatment and tissue engineering in both of neural and bone damages repair.
Anderson DJ,Gage FH, Weissman IL. Can stem cells cross lineage boundaries. Natmed. 2001;7:393-395.
Wang J, Wei X, Ling J, Huang Y, Huo Y, Zhou Y. The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells. BiochemBiophys Res Commun. 2010;400(3):334-9.
Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, et al. SHED: stem cells from human exfoliated deciduous teeth. ProcNatlAcadSci U S A. 2003;100(10):5807-12.
Wagers AJ, Weissman IL. Plasticity of adult stem cells. Cell. 2004;116(5):639-48.
Conley BJ, Young JC, Trounson AO, Mollard R. Derivation, propagation and differentiation of human embryonic stem cells. Int J Biochem Cell Biol. 2004;36(4):555-67.
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282(5391):1145-7.
Namvaran AA, Tavakkoli FG. The effect of Salvia officinalishydroalcoholic extract on PTZ-induced seizure threshold in Vincristine injected mice. J ShahrekordUniv Med Sci. 2012;13(6):47-55.
Takeda T, Tezuka Y, Horiuchi M, Hosono K, Lida K, Hatakeyama D, et al. Characterization of dental pulp stem cells of human tooth germs. J Dent Res. 2008;87(7):676-81.
Agha-Hosseini F, Jahani MA, Jahani M, Mirzaii-Dizgah I, Ali-Moghaddam K. In vitro isolation of stem cells derived from human dental pulp. Clin Transplant. 2010;24(2):E23-8.
Mao JJ, Giannobile WV, Helms JA, Hollister SJ, Krebsbach PH, Longaker MT, et al. Craniofacial tissue engineering by stem cells. J Dent Res. 2006;85(11):966-79.
Caplan AI. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol. 2007;213(2):341-7.
Korbling M, Estrov Z. Adult stem cells for tissue repair - a new therapeutic concept? N Engl J Med. 2003;349(6):570-82.
Eslaminejad MB, Taghiyar L. Study of the structure of canine mesenchymal stem cell osteogenic culture. Anatomia, histologia, embryologia. 2010;39(5):446-55.
Eslaminejad MB, Jafarian M, Khojasteh A, Mashhadi AF, et al. In vivo Bone Formation by Canine Mesenchymal Stem Cells Loaded onto HA/TCP Scaffolds: Qualitative and Quantitative Analysis, Yakhteh Medical Journal. 2008; 10(3): 205-212.
Jabbarpour BM, Mahdavi N. Studing the effect of mitogen activated protein kinase inhibitor (MAPKI) on mesenchymal stem cells in vitro, Pharmaceutical Science. 2005; 3: 39-44.
Hartmann C. A Wnt canon orchestrating osteoblastogenesis. Trends Cell Biol. 2006;16(3):151-8.
Dalamagkas K, Tsintou M, Seifalian A. Advances in peripheral nervous system regenerative therapeutic strategies: A biomaterials approach. Materials science & engineering C, Materials for biological applications. 2016;65:425-32.
Kadkhoda Z, Safarpour A, Azmoodeh F, Adibi S, Khoshzaban A, Bahrami N. Histopathological Comparison between Bone Marrow- and Periodontium-derived Stem Cells for Bone Regeneration in Rabbit Calvaria. Int J Organ Transplant Med. 2016;7(1):9-18.
Gregory CA, Gunn WG, Peister A, Prockop DJ. An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction, Analytical Biochemistry. 2004; 329: 77-84.
Hare JM, DiFede DL, Rieger AC, Florea V, Landin AM, El-Khorazaty J, et al. Randomized comparison of allogeneic versus autologous mesenchymal stem cells for nonischemic dilated cardiomyopathy: POSEIDON-DCM trial. Journal of the American College of Cardiology. 2017;69(5):526-37.
Tomic S, Djokic J, Vasilijic S, Vucevic D, Todorovic V, Supic G, et al. Immunomodulatory properties of mesenchymal stem cells derived from dental pulp and dental follicle are susceptible to activation by toll-like receptor agonists. Stem Cells Dev. 2011;20(4):695-708.
Mao JJ, Giannobile WV, Helms JA, Hollister SJ, Krebsbach PH, Longaker MT, et al. Craniofacial tissue engineering by stem cells. Journal of dental research. 2006;85(11):966-79.
Sharma RR, Pollock K, Hubel A, McKenna D. Mesenchymal stem or stromal cells: a review of clinical applications and manufacturing practices. Transfusion. 2014;54(5):1418-37.
Yalvac ME, Ramazanoglu M, Rizvanov AA, Sahin F, Bayrak OF, Salli U, et al. Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implication in neo-vascularization, osteo-,adipo-and neurogenesis. Pharmacogenomics J. 2010;10(2):105-13.
Mashadi Abbas, Mojarrad S, Yadegary Z, Sharifi B. Isolation and characterization of mesenchymal stem cells derived from the dental pulp and follicle.J of Dent Med-Tehran University of Medical Sciences. 2011;24(2):69-76
Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. ProcNatlAcadSci USA 2000;97:13625e30.
Gregory CA, Gunn WG, Peister A, Prockop DJ. An Alizarin red-based assay of mineralization byadherent cells in culture: comparison withcetylpyridinium chloride extraction, AnalyticalBiochemistry. 2004; 329: 77-84.
Zaminy A, RagerdiKashani I, Barbarestani M, Hedayatpour A, et al. Melatonin Influences theProliferative and Differentiative Activity of RatAdipose-Derived Stem Cells, Yakhteh MedicalJournal. 2008; 10(1): 25-32.
Hartmann C. A Wnt canon orchestratingosteoblastogenesis, Trends in cell biology. 2006; 16:151-158.
George R, Beck Jr, Zerler B, Moran E.Phosphate is a specific signal for induction ofosteopontin gene expression, PNAS. 2000; 97(15):8352–8357.
Dvorak MM, Siddiqua A, Ward DT, CarterDH, et al. Physiological changes in extracellularcalcium concentration directly control osteoblastfunction in the absence of calciotropic hormones.PNAS. 2004; 101(14): 5140-5145.
Venkata Ramesh Dasari, Krishna Kumar Veeravalli, Dzung H Dinh. Mesenchymal stem cells in the treatment of spinal cord injuries: A review. World J Stem Cells 2014 April 26; 6(2): 120-133
Abnoosi MH, Dehdehi LM. Study of Morphology and Biochemistry of Rat Bone Marrow Mesenchymal Stem Cells Before and After Osteogenic Differentiation: A Comparative Study. Journal of Cell & Tissue. 2012; 3(2):103-111.
Mori G, Ballini A, Carbone C, Oranger A, Brunetti G, Di Benedetto A, et al. Osteogenic differentiation of dental follicle stem cells. Int J Med Sci. 2012;9(6):480-7.
Chang CC, Chang KC, Tsai SJ, Chang HH, Lin CP. Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media. Journal of the Formosan Medical Association = Taiwan yizhi. 2014;113(12):956-65.
Ullah I, Subbarao RB, Kim EJ, Bharti D, Jang SJ, Park JS, et al. In vitro comparative analysis of human dental stem cells from a single donor and its neuronal differentiation potential evaluated by electrophysiology. Life Sci. 2016;154:39-51.
BahramiN, ShiraniGh, ShayestehSoleymaniY, ManafiZ, MohajeryF. Neural Differentiation Potential of Stem Cells Derived from Dental Follicle and Periodontal Ligament stem cells.
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