The evaluation and comparison of the elastic modulus of mechanically motivated and pure rabbit mesenchymal stem cell with mature chondrocytes

Shahrokh Shojaei, Amir Shirazian, Azadeh Jahanbakhsh, Hanie Kavand, Nooshin Haghighipour

Abstract


Different cells are located in different anatomical location of the body and consequently they are exposed to different mechanical forces. Chondrocytes which form the cartilage tissue are located in articular joints like knee and each day they are influenced by intermittent hydrostatic pressure of thousands of times. Mechanical stimulations of cells have vital contributions on acquisition of functional characteristics and elasticity of the cells which is define by elastic modulus is one the most imperative mechanical properties. The arrangement and organization of actin fibers in cytoskeleton can determine the elasticity of cells and it has been illustrated that it plays a pivotal role in some important cellular activities such as motility or cell to cell interaction. In this research the stem cells and mature chondrocytes were extracted from rabbit adipose and cartilage tissue respectively and cultured till the passage of three. A unique bioreactor which was previously designed and manufactured was used to apply intermittent hydrostatic pressure (0-1 MPa, 0.5 Hz) to Rabbit Mesenchymal Stem Cells (RMSCs). After the application of forces the elastic modulus of different groups of cells were assessed and compared by atomic force microscopy. The results demonstrated that application of hydrostatic pressure can modify the elastic modulus of stem cells and make them more resemble to mature chondrocytes. The result of this paper can be important for applications of cartilage tissue engineering.


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