Optimizing the Proliferation and Differentiation of Mesenchymal Stem Cells by Using PLLA Nanofibers for Epidermis Layer Repair

  • Ehsan Seyedjafari
  • Arsalan Jalili
  • Kaykhosro Moridi
  • Zakieh Mokhames
Keywords: Nanofibrous PLLA Scaffolds, Electrospining, skin epidermal keratinocytes, Mesenchymal stem cell

Abstract

Background: Tissue engineering has attracted a lot of attention, especially regarding wound and skin damage repair. PLLA is a biocompatible and biodegradable polymer. It has excellent mechanical properties and is able to support cell proliferation and growth. Electrospinnig is a fabrication method which has gained a lot of attention over the past years. As a result, a lot of studies have been carried out using it.

Methods and Materials: -In the present study, the potential of PLLA fabricated scaffolds is evaluated. Scaffolds were fabricated by electrospinning. After fabrication they were then tested for toxicity, using MTT assay. After confirming their safety, their potential for cell and stem cell proliferation and growth were examined. Additionally, another step was taken and mesenchymal stem cells were differentiated to skin cells on PLLA nanofibers. After 21 days of differentiation, PCR, SEM imaging were performed.

Results: SEM imaging showed a clear transformation of stem cells. Furthermore, PCR analysis confirmed the differentiation of relevant genes.

Conclusion: Our results undoubtedly confirmed the potential of PLLA in tissue engineering. It yielded a feasible nanoscaffold, with great mechanical properties. Furthermore, it helped support cell and stem cell growth, as well as skin differentiation of stem cells.

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Published
2020-06-17
Section
Original Articles