Optimizing the Proliferation and Differentiation of Mesenchymal Stem Cells by Using PLLA Nanofibers for Epidermis Layer Repair

  • Ehsan Seyedjafari
  • Arsalan Jalili
  • Kaykhosro Moridi
  • Zakieh Mokhames
Keywords: Nanofibrous PLLA Scaffolds, Electrospining, skin epidermal keratinocytes, Mesenchymal stem cell


Background: Tissue engineering has attracted a lot of attention, especially regarding wound and skin damage repair. PLLA is a biocompatible and biodegradable polymer. It has excellent mechanical properties and is able to support cell proliferation and growth. Electrospinnig is a fabrication method which has gained a lot of attention over the past years. As a result, a lot of studies have been carried out using it.

Methods and Materials: -In the present study, the potential of PLLA fabricated scaffolds is evaluated. Scaffolds were fabricated by electrospinning. After fabrication they were then tested for toxicity, using MTT assay. After confirming their safety, their potential for cell and stem cell proliferation and growth were examined. Additionally, another step was taken and mesenchymal stem cells were differentiated to skin cells on PLLA nanofibers. After 21 days of differentiation, PCR, SEM imaging were performed.

Results: SEM imaging showed a clear transformation of stem cells. Furthermore, PCR analysis confirmed the differentiation of relevant genes.

Conclusion: Our results undoubtedly confirmed the potential of PLLA in tissue engineering. It yielded a feasible nanoscaffold, with great mechanical properties. Furthermore, it helped support cell and stem cell growth, as well as skin differentiation of stem cells.


Atul A. Chaudhari, Komal Vig, Dieudonné Radé, Baganizi,Rajnish, Sahu,Saurabh, Dixit,Vida Dennis, Shree Ram Singh, Shreekumar R. Pillai. Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review, Int. J. Mol. Sci. 2016, 17(12), 1974

Azizeh-Mitra Yousefi, Paul F. James, Rosa Akbarzadeh, Aswati Subramanian, Conor Flavin, and Hassane Oudadesse. Prospect of Stem Cells in Bone Tissue Engineering: A Review, Stem Cells International, Volume 2016, Article ID 6180487

Nikolaos K. Paschos, Wendy E. Brown, Rajalakshmanan Eswaramoorthy, Jerry C. Hu, Kyriacos A. Athanasiou. Advances in tissue engineering through stem cell‐based co‐culture, Journal of Tissue Engineering and Regenerative Volume9, Issue5, May 2015, Pages 488-503

Ali Khademhosseini & Robert Langer. A decade of progress in tissue engineering, Nature Protocols volume11, pages1775–1781 (2016)

Neha Maheshwari , Muktika Tekade , Yashu Chourasiya , Mukesh Chandra Sharma , Pran Kishore Deb and Rakesh K. Tekade. Nanotechnology in Tissue Engineering, Advances in Pharmaceutical Product Development and Research2019, Pages 225-261.

Tao Jianga, Erica J. Carbonea, Kevin W.-H. Loa, Cato T. Laurencinb. Electrospinning of polymer nanofibers for tissue regeneration, Progress in Polymer Science Volume 46, July 2015, Pages 1-24

Jiajia Xue, Jingwei Xie, Wenying Liu, and Younan Xia. Electrospun Nanofibers: New Concepts, Materials, and Applications, Acc. Chem. Res.2017508 1976-1987

Mohammadali Sheikholeslam, Meghan E. E. Wright, Marc G. Jeschke, Saeid Amini‐Nik. Biomaterials for Skin Substitutes, Volume7, Issue5, March 7, 2018

Agathe Grémare,Vera Guduric, Reine Bareille, Valérie Heroguez, Simon Latour, Nicolas L'heureux, Jean‐Christophe Fricain, Sylvain Catros, Damien Le Nihouannen. Characterization of printed PLA scaffolds for bone tissue engineering, Journal of Biomedical Materials Research Part A, Volume106, Issue4 April 2018 Pages 887-894.

Fatemeh Asghari,Mohammad Samiei,Khosro Adibkia,Abolfazl Akbarzadeh &Soodabeh Davaran. Biodegradable and biocompatible polymers for tissue engineering application: a review, Pages 185-192, published online: 28 Feb 2016

Marco Santoro, Sarita R. Shah, Jennifer L. Walker, Antonios G. Mikos. Poly(lactic acid) nanofibrous scaffolds for tissue engineering, Advanced Drug Delivery Reviews, Volume 107, 15 December 2016, Pages 206-212.

Yinxian Yu, Sha Hua, Mengkai Yang, Zeze Fu, Songsong Teng, Kerun Niu, Qinghua Zhao, Chengqing Yi. Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold, RSC Advances, Issue 112, 2016.

YanhengGuo, Jordana Gilbert-Honick, Sarah M.Somers, Hai-Quan Mao, Warren L.Grayson. Modified cell-electrospinning for 3D myogenesis of C2C12s in aligned fibrin microfiber bundles, Biochemical and Biophysical Research Communications, 22 June 2019.

Naagarajan Narayanan, Chunhui Jiang, Gozde Uzunalli,Shalumon Kottappally Thankappan, Cato T. Laurencin, Meng Deng. Polymeric Electrospinning for Musculoskeletal Regenerative Engineering, June 2016, Volume 2, Issue 2, pp 69–84.

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