Evaluation of Biocompatible Bioglass/Gelatin ScaffoldEnhanced by Mesenchymal Stem Cell for Bone Tissue Regeneration

  • Elham Alsadat Hosseini Aghozbeni
  • Abbas Ali ImaniFooladi
  • Mohammad Reza Nourani
Keywords: Bioglass, MSCs, scaffold, bone, regeneration


In this study, we are focused on developing cell–scaffold constructs to improve bone growth and bone healing. Rat MSCs were isolated from the femurs of rats. The bone marrow suspensions were cultured in the medium consisted of Dulbecco modified Eagle medium supplemented with fetal bovine serum and penicillin–streptomycin. Bioglassnano-composite (BG) scaffold was made through Sol-Gel methodand was evaluated by in vitro cytotoxicity.Osteogenic materials added tothe culture medium and occurrence of differentiation examined by alizarin red staining. The results revealed that BG scaffolds has no toxicity and is biocompatible, following alizarin red staining thered color demonstrated the mineralizing areas of cultures. The present study demonstrates that rMSCs enable to differentiate to bonecell on the Nano bioglass scaffolds.


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