Evaluation of Biocompatible Bioglass/Gelatin ScaffoldEnhanced by Mesenchymal Stem Cell for Bone Tissue Regeneration
DOI:
https://doi.org/10.22034/JATE.2014.6Keywords:
Bioglass, MSCs, scaffold, bone, regenerationAbstract
In this study, we are focused on developing cell–scaffold constructs to improve bone growth and bone healing. Rat MSCs were isolated from the femurs of rats. The bone marrow suspensions were cultured in the medium consisted of Dulbecco modified Eagle medium supplemented with fetal bovine serum and penicillin–streptomycin. Bioglassnano-composite (BG) scaffold was made through Sol-Gel methodand was evaluated by in vitro cytotoxicity.Osteogenic materials added tothe culture medium and occurrence of differentiation examined by alizarin red staining. The results revealed that BG scaffolds has no toxicity and is biocompatible, following alizarin red staining thered color demonstrated the mineralizing areas of cultures. The present study demonstrates that rMSCs enable to differentiate to bonecell on the Nano bioglass scaffolds.
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7. MONNIPHA SILA-ASNA, AHNOND BUNYARATVEJ, SAKAN MAEDA, HIROMICHI KITAGUCHI, and NARONG BUNYARATAVEJ, "Osteoblast Differentiation and Bone Formation Gene Expression in Strontium-inducing Bone Marrow Mesenchymal Stem Cell," Kobe J. Med.Sci,2007, pp. 25-35.
8. QISHENG TU, M.D.,Ph.D., PALOMA VALVERDE,Ph.D., SHU LI,D.D.S.,Ph.D., JIN ZHANG, D.D.S., PISHAN YANG,D.D.S., Ph.D., JAKE CHEN,D.D.S.,Ph.D, "Osterix Overexpression in Mesenchymal Stem Cells Stimulates Healing of Critical-Sized Defects in Murine Calvarial Bone," Tissue Eng, 2007,pp. 13(10):2431-2440.
9. ForoughHafezi. FatemehHosseinnejad. Abbas Ali ImaniFooladi. SoroushMohitMafi. AfsanehAmiri. Mohammad Reza Nourani, "Transplantation of nano-bioglass/gelatin scaffold in a non-autogenous setting for bone regeneration in a rabbite ulna," J Mater Sci:Mater Med, 2012.
10. Azami M, Rabiee M, Moztarzadeh F, "Glutaraldehydecrosslinked gelatin/hydroxyapatite nanocomposite scaffold, engineered via compound techniques," Polym Compos, 2010,pp. 31(12):2112-20.
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12. Fassina L, Saino E, Visai L, Avanzini MA, Cusella De Angelis MG, Benazzo F, Van Vlierberghe S, Dubruel P, Magenes G, "Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells," ConfProc IEEE Eng Med BiolSoc, 2010,pp. 247-50.
13. Chen Zong, DetingXue, Wenji Yuan, Wei Wang, Dan Shen, Xiangmin Tong, et al, "Reconstruction of rat carvarlal defects with human mesenshymal stem cells in and osteoblast – like cells in poly-lactic-co-glycolic acid scaffolds," European Cells and Materials, 2010,pp. 109-120.
14. Xiaoling Zhang, Tingting Tang, Qin Shi, Julio C. Fernandes, Kerong Dai, "The immunologenic properties of undifferentiated and osteogenic differentiated mouse mesenchymal stem cells and its potential application in bone regeneration," Immunobiology, 2009,pp. 179-186.
15. A.J.Salgada, O.P.Coutinho, R.L.Reis, "Bone tissue engineering: state of the art and future trends," MacromolBiosci, 2004,pp. 743-765.
16. D.W.Hutmacher, "Scaffolds in tissue engineering bone and cartilage," Biomaterials, 2000,pp. 2529-2543.
17. Dror Ben-David, TaliA.Kizhner, Thomas Kohler, Ralph Muller, ErellaLivne, SamerSrouji, "Cell-scaffold transplant of hydrogel seeded with rat bone marrow progenitors for bone regeneration," Journal of Cranio-Maxillo-Facial Surgery, 2011,pp. 364-371.
18. Stock SR, "X-ray microtomography of materials," Int Mater Rev, 1999,pp. 44:141-64.
19. Miao, T.M.O.S.A.X, "Preparation and characterisation of PLGA-coated porous bioactive glass ceramic scaffolds for subchondral bone tissue engineering," Ceramic Transactions, 2008.
20. J.L.Milan, Josep A. Planell and Damien Lacroix, "Simulation of bone tissue formation within a porous scaffold under dynamic compression," Biomech Model Mechanobiol, 2010.
21. Ali Doostmohammadi, Ahmad Monshi, RasoulSalehi, MohimaniammadHosseinFathi, EhsanSeyedjafari, Abbas Shafiee, Masoud Sole, "CYTOTOXICITY EVALUATION OF 63S BIOACTIVE GLASS AND BONE-DERIVED HYDROXYAPATITE PARTICLES USING HUMAN BONE-MARROW STEM CELLS," Biomed Pap Med FacUnivPalacky Olomouc Czech Repub, 2011,pp. 155(4):323-326.
22. MohamadrezaBaghabanEslaminejad, AghbibiNikmahzar, Leila Taghiyar, SamadNadri, MohamadMassumi, "Murine mesenchymal stem cells isolated by low density primary culture system," Develop. Growth Differ, 2006,pp. 361-370.
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Published
2019-12-05
How to Cite
Hosseini Aghozbeni , E. A., ImaniFooladi , A. A., & Nourani , M. R. (2019). Evaluation of Biocompatible Bioglass/Gelatin ScaffoldEnhanced by Mesenchymal Stem Cell for Bone Tissue Regeneration. The Journal of Applied Tissue Engineering, 1(1), 14–18. https://doi.org/10.22034/JATE.2014.6
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