Evaluation the cytotoxic/viability effect of gelatin/bioactive glass conduits by MTT assay
DOI:
https://doi.org/10.22034/JATE.2014.8Keywords:
bioactive glass, Conduit,Cytotoxicity effects, tissue engineering.Abstract
Peripheral nerve injury can successfully heal,if an appropriate environment and route be provided.This study was designed to develop a novel gelatin/ bioactive glass conduits using freeze-drying technique. The gelatin/ bioactive glass conduits characterized using Fourier transform infrared spectroscopy (FTIR).The surface morphology of the Nanocompositewere investigated through scanning electron microscopy (SEM).Cytotoxicity Evaluation show that the CHO cells had attached and proliferated in the surroundings of the conduit. Biocomptibilitywas assessed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenylte-2H-tetrazolium bromide) assay which showed that gelatin/bioactive glass conduits had good cytocompatibilit.
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2. Rezwan, K., et al., Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials, 2006. 27(18): p. 3413-31.
3. Guarino, V., F. Causa, and L. Ambrosio, Bioactive scaffolds for bone and ligament tissue. Expert Rev Med Devices, 2007. 4(3): p. 405-18.
4. Liu, X., et al., Biomimetic nanofibrous gelatin/ apatite composite scaffolds for bone tissue engineering. Biomaterials, 2009: p. 2252–2258.
5. Mozafari, M., et al., Development of macroporous nanocomposite scaffolds of gelatin/bioactiveglass prepared through layer solvent casting combined with laminationtechnique for bone tissue engineeringCeramics International 2010: p. 2431-2439.
6. picot, J., human cell culture protocol. 2004, CA, USA
7. Fassina L, S.E., Visai L, Avanzini MA, Cusella De Angelis MG, Benazzo F, Van Vlierberghe S, Dubruel P, Magenes G., Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells. Conf Proc IEEE Eng Med Biol Soc., 2010: p. 247-50.
8. Azami, M., F. Moztarzadeh, and M. Tahriri, Preparation characterization and mechanical properties of controlled porous gelatin/hydroxyapatite nanocomposite through layer solvent casting combined with freeze-drying and lamination techniques. Porous Mater, 2009.
9. Mortazavi, V., et al., Antibacterial effects of sol-gel-derived bioactive glass nanoparticle on aerobic bacteria. Biomed Mater Res A, 2010: p. 160-8.
10. Yang, Y., et al., Nerve conduits based on immobilization of nerve growth factor onto modified chitosan by using genipin as a crosslinking agent. Pharmaceutics and Biopharmaceutics, 2011: p. 519–525.
11. S, J., et al., Biocompatibility and cytotoxicity of bioglass-ceramic composite with various P2O5 content in Li2O-SiO2-CaO-CaF2-P2O5 system on fibroblast cell lines. Acta Chimica Slovaca, 2011.
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Published
2019-12-05
How to Cite
Masoumeh , F. K., Kamali , M., Nourani , M. R., & Abbas Ali , I. F. (2019). Evaluation the cytotoxic/viability effect of gelatin/bioactive glass conduits by MTT assay. The Journal of Applied Tissue Engineering, 1(1), 22–25. https://doi.org/10.22034/JATE.2014.8
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